Sequence of a cDNA coding for human IRF-2.

Nucleic Acids Research Sequence of a cDNA coding for human IRF-2 Susumu Itoh*, Hisashi Harada, Takashi Fujita, Tsutomu Mimura and Tadatsugu Taniguchi Institute for Molecular and Cellular Biology, Osaka University, Yamadaoka 1-3, Suita-Shi, Osaka 565 and 'Faculty of Pharmaceutical Science, Osaka University, Yamadaoka 1-6, Suita-shi, Osaka 565, Japan Submitted September II, 1989 EMBL accession no. XI5949 We have previously isolated a cDNA done, a mouse interferon regulatory factor-2 (IRF-2) (Harada, H , Fujita, T., Miyamoto, ivL, Kimura, Y., Maruyama, M, Furia, A., Miyata, T. and Taniguchi, T., 1989, Cell, 5J, 729-739). We have isolated a human IRF-2 cDNA coding for human IRF-2 by crosshybridization with the mouse IRF-2 full length cDNA probe, using the human T cell line, Jurkat-111 cDNA library. One of the positive clones, pfflRF4S-51, was sequenced. This cDNA includes 98 bp of 5"untranslated, 1047 bp of coding and 999 bp of 3'-untranslated regions. The deduced amino acid sequence is the 349 amino acid protein and this protein shows 93.5% homology, compared with mouse IRF-2. Furthermore, the amino terminal hah' of human IRF-2 from 1 to 113 amino acid has 73% homology to human IRF-1 (Maruyama, M., Fujita, T. and Taniguchi, T., Nucleic Acidi Research, 1989, 7,3292).